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Zebrafish Module: Zebrafish Development and Disease

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Objectives [top]

  1. Understand the basics of blood glucose regulation in normal and diabetic states.
  2. Understand advantages of using a genetic model system.
  3. Familiarization with different embryonic and larval stages of zebrafish.
  4. Understand how genetic alterations can impact pancreatic development.

Activities [top]

  1. Discuss pancreas physiology and diseases, using the zebrafish as a developmental genetics model, and loss of function studies in zebrafish.
  2. Examine adult zebrafish strains and learn the differences between male and female fish.
  3. Examine pre-mounted, staged zebrafish embryos using brightfield microscopy.
  4. Collect fertilized eggs from a zebrafish mating pair.
  5. Mount wild type and morpholino-injected embryos (morphants) in 2% methylcellulose.
  6. Examine and document wild type and morphant embryos using epi-fluorescence microscope.

Questions [top]

  1. What is the difference between an endocrine and an exocrine gland?
  2. Which pancreatic cells secrete insulin?
  3. What triggers insulin release, and what is the function of insulin?
  4. What are three characteristics that make the zebrafish a useful model system?
  5. When does pancreas development begin in humans? in mice? in zebrafish?
  6. Name two ways to generate loss-of-function in zebrafish embryos.

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