Zebrafish Module: Zebrafish Development and Disease
- Understand the basics of blood glucose regulation in normal and diabetic states.
- Understand advantages of using a genetic model system.
- Familiarization with different embryonic and larval stages of zebrafish.
- Understand how genetic alterations can impact pancreatic development.
- Discuss pancreas physiology and diseases, using the zebrafish as a developmental genetics model, and loss of function studies in zebrafish.
- Examine adult zebrafish strains and learn the differences between male and female fish.
- Examine pre-mounted, staged zebrafish embryos using brightfield microscopy.
- Collect fertilized eggs from a zebrafish mating pair.
- Mount wild type and morpholino-injected embryos (morphants) in 2% methylcellulose.
- Examine and document wild type and morphant embryos using epi-fluorescence microscope.
- What is the difference between an endocrine and an exocrine gland?
- Which pancreatic cells secrete insulin?
- What triggers insulin release, and what is the function of insulin?
- What are three characteristics that make the zebrafish a useful model system?
- When does pancreas development begin in humans? in mice? in zebrafish?
- Name two ways to generate loss-of-function in zebrafish embryos.
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