Dissecting Diabetes Module
Upon completion of this module, you will be able to:
- Know the difference between the endocrine and exocrine pancreas.
- Identify the islets of Langerhans and know the function of its cells.
- Understand how environmental or genetic factors influence islets and diabetes.
- Discuss how microscopy can be used for research.
- Discuss glucose metabolism, diabetes, the pancreas, and the islets of Langerhans.
- Observe mouse models for diabetes research.
- Perform staining of pancreas sections of microscope slides with hematoxylin and eosin (H&E).
- Xylene 5 min 2X
- 100% EtOH 1 min
- 95% EtOH 1 min
- 80% EtOH 1 min
- 70% EtOH 1 min
Staining [You will start here.]
- Hematoxylin 2 min
- Rinse dH2O
- Acid Alcohol 2 sec (.1% HCL in 50% EtOH)
- Rinse Tap Water 5 min
- Eosin 1 min
- Rinse in a few quick changes of dH2O
- 95% EtOH about 1 min as needed
- 100% EtOH 1 min
- Xylene 2 min 2X
- Coverslip [Your instructors will do this step.]
- Observe H&E staining of pancreas sections with a microscope. [You will start again here.]
- Discuss other ways to stain the pancreas and identify the specific cell types of the islet.
- Recap how microscopy can be used to observe the progression of diabetes.
- What cells make up the islets of Langerhans and what do they do?
- Are islets of Langerhans consistent in size and shape or do they vary?
- What percentage of the pancreas contains islets?
- Can environmental factors change the appearance and size of islets?
Interesting websites [top]
http://diabetes.niddk.nih.gov/ - Site from the National Instite of Health contains statistics about diabetes and ample introductory material about the disease.
http://www.diabetes.org/ - Site from the American Diabetes Association provides the basics about diabetes as well as news and research related to diabetes.
Note: Images from the following sites: