Role of Angiogenesis in Diseases
- Discuss hematopoietic cells and the bone marrow microenvironment, and:
- assays to measure hematopoietic progenitors;
- assays to measure endothelial cell (EC) functions (morphogenesis, proliferation).
- Discuss involvement of endothelial cells in cancer development – tumor angiogenesis.
- Aseptically harvest, centrifuge, resuspend cells in Matrigel or methylcellulose.
- Discuss the use of specific growth factors for differentiation of a specific lineage.
- Discuss formation of hematopoietic colonies, endothelial cell morphogenesis (in vitro capillary-like structures), formation of cellular networks and 3D structures.
- Discuss use of Matrigel assay for screen for anti-angiogenic drugs.
- Observe EC monolayer under microscope and discuss how EC were isolated.
- Learn sterile techniques used for tissue culture preparation.
- Aseptically harvest ECs from plates provided. Cells will be placed in tubes and centrifuged.
- Discard media, resuspend cell pellet, and count cells. Resuspend cells in Matrigel and plate them into a 48 well tissue plate.
- Observe capillary-like structures formed in Matrigel using the microscope, and take pictures; observe the effect of specific drug on capillary-like structure formation.
- Why do EC display a different behavior in Matrigel than when cultured in plastic?
- Which properties distinguish EC cells from the other adherent cell lineages in Matrigel?
- What growth factors are necessary to support EC functions?
- Why use drugs that inhibit specific signaling molecules to inhibit EC and tumor angiogenesis?